Carl Wu

Carl Wu

netbet casino review

Contact Information

netbet online sports bettingEukaryotic chromatin structure and function. Biochemistry of nucleosome remodeling and gene transcription, and live cell-single molecule imaging of histone and nuclear protein dynamics

Carl Wu joined Johns netbet casino reviewHopkins University in 2016 as Bloomberg Distinguished Professor with appointments in the Department of Biology, and the Department of Molecular Biology and Genetics. He received his Ph.D. in Biology at Harvard University and did post-doctoral work as a Harvard Junior Fellow. He was a Principal Investigator at the National Cancer Institute for 30 years, and a member of the Transcription Imaging Consortium at HHMI-Janelia (2012 - 2016). He is a member of the US National Academy of Sciences, National Academy of Medicine, American Academy of Arts and Sciences, Academia Sinica, and the European Molecular Biology Organization.

Chromatin architecture and histone dynamics at eukaryotic gene promoters

Our lab is interested in understanding the structure and function of chromatin, which has a key role in regulating essentially all chromosome activities. We are currently studying the biochemistry of the conserved histone variant, H2A.Z, which is uniquely incorporated at all eukaryotic promoters and enhancers genome-wide, and has an important role in the control of transcription. By elucidating the mechanisms underlying site-specific incorporation of H2A.Z into nucleosomes and the function of nucleosomal H2A.Z in transcription, we hope to acquire general insights on promoter and netbet casino reviewenhancer function in health and disease. We primarily use the budding yeast model organism because of its efficient molecular genetics and biochemistry, and evolutionary conservation of fundamental mechanisms of gene regulation.

Histone H2A.Z is incorporated into nucleosomes by SWR1, a 14-component, 1-megadalton chromatin remodeler conserved from yeast to humans. SWR1 has two distinct substrates – the canonical nucleosome and the variant H2A.Z-H2B dimer. In the histone exchange reaction, SWR1 mediates ATP-dependent eviction of one nucleosomal histone H2A-H2B dimer coupled with deposition of H2A.Z-H2B, followed by a second histone dimer exchange. Biochemical and structural studies have elucidated how SWR1 captures the H2A.Z-H2B dimer and targets nucleosomes flanking free promoter DNA - both actions primarily through the Swc2/YL1 subunit - but little is known about the molecular mechanism of ATP-dependent histone exchange. To answer this question, we take a multi-disciplinary approach, using the tools of biochemistry, cell and molecular biology, combined with structural biology and single-molecule biophysics. In collaboration with T.J. Ha’s lab, we are developing a single-molecule, real-time fluorescence colocalization and fluorescence netbet casino reviewresonance energy transfer approach to detect reaction intermediates and measure their lifetimes during histone exchange.

We have also embarked on a new venture using live-cell, single-molecule fluorescence microscopy to image the dynamics of transcription factors and chromatin-based enzymes such as SWR1. The live-cell approach avoids fixation artifacts, measures the movements of single molecules at high spatiotemporal resolution, and should elucidate how different factors gain access to chromatin in vivo. By comparing diffusive parameters between wild-type cells and those exhibiting defects in the epigenetic landscape, we will gain new insights on the role of chromatin organization and dynamics in transcription.

We are additionally interested in the unusual biochemistry of variant nucleosomes at chromosome centromeres. Unlike nucleosome remodeling at gene promoters, nucleosome stability without turnover at centromeres is obligatory for kinetochore assembly during cell division. Yeast centromeres assemble nucleosomes harboring a histone H3 variant called Cse4/ CENP-A. Both Cse4 histone residues and AT-rich centromere DNA composition are important for recruiting CENP-C, a key kinetochore protein, and thus histones and DNA together have netbet casino reviewa role in centromere specification.

Additional research can be found on the Wu Lab website

Selected publications

Nguyen VQ, Ranjan A, Stengel F, Wei D, Aebersold R, Wu C, Leschziner AE. Molecular Architecture of the ATP-Dependent Chromatin-Remodeling Complex SWR1. Cell 154:1220-31 (2013).

Ranjan A, Mizuguchi G, Fitzgerald PC, Wei D, Wang F, Huang Y, Luk E, Woodcock CL, Wu C. Nucleosome-free Region Dominates Histone Acetylation in Targeting SWR1 to Promoters for H2A.Z Replacement. Cell 154:1232-45 (2013).

Hong J, Feng H, Wang F, Ranjan A, Chen J, Jiang J, Ghirlando R, Xiao TS, Wu C, Bai Y. The Catalytic Subunit of the SWR1 Remodeler Is a Histone Chaperone for the H2A.Z-H2B Dimer. Mol Cell. 53:498-505 (2014).

Wisniewski J, Hajj B, Chen J, Mizuguchi G, Xiao H, Wei D, Dahan M, Wu C. Imaging the fate of histone Cse4 reveals de novo replacement in S phase and subsequent stable residence at centromeres. eLIFE May 20 (2014).

Hajj B, Wisniewski J, El Beheiry M, Chen J, Revyakin A, Wu C, Dahan M. Whole-cell, multicolor super-resolution imaging using volumetric multifocus microscopy. Proc Natl Acad Sci U S A. 111:17480-5 (2014).

Ranjan A, Wang F, Wei D, Huang Y, Wu C. Nucleosome H2A histone fold and DNA elements activate SWR1-mediated H2A.Z replacement eLIFE June 27 (2015).

Abrahamsson S, Ilic R, Wisniewski J, Mehl B, Yu L, Chen L, Davanco M, Oudjedi L, Fiche J, NetBet live casinoHajj B, Jin X, Pulupa J, Cho C, Mir M, El Beheiry M, Darzacq X, Nollmann M, Dahan M, Wu C, Lionnet T, Liddle A, Bargmann C. Multifocus microscopy with precise color multi-phase diffractive optics applied in functional neuronal imaging. Biomedical Optics Express 7(3):855-869 (2016).

Liang X, Shan S, Pan L, Zhao J, Ranjan A, Wang F, Zhang Z, Huang Y, Feng H, Wei D, Huang L, Liu X, Zhong Q, Lou J, Li G, Wu C, Zhou, Z. Structural basis of H2A.Z recognition by SRCAP chromatin remodelling subunit YL1. Nat Struct Mol Biol. 23(4):317-23. Epub Mar 14 (2016)

Wang F, Ranjan A, Wei D, Wu C. Comment on "A histone acetylation switch regulates H2A.Z deposition by the SWR-C remodeling enzyme". Science 353(6297):358, PMID: 27463665 (2016).

Shaytan AK, Xiao H, Armeev GA, Wu C, Landsman D, Panchenko AR. Hydroxyl-radical footprinting combined with molecular modeling identifies unique features of DNA conformation and nucleosome positioning. Nucleic Acids Res. Sep 19; 45(16):9229-9243, PMID 28934480 (2017).

Xiao H, Wang F, Wisniewski J, Shaytan AK, Ghirlando R, Fitzgerald P, Huang Y, Wei D, Li S, Landsman D, Panchenko AR, Wu C. Molecular basis of CENP-C association with the CENP-A nucleosome at yeast centromeres. Genes Dev. Oct 26 Epub ahead of print, PMID 29074736 (2017).

All lab staff is listed on the Wu Lab website